B-Cell Linker Protein (BLNK) (His tag)
-
- Target See all B-Cell Linker (BLNK) Proteins
- B-Cell Linker (BLNK)
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This B-Cell Linker protein is labelled with His tag.
- Purpose
- Recombinant Human BLNK/Ly-57 Protein (His Tag)
- Sequence
- Met1-Ser456
- Characteristics
- Recombinant Human BLNK is produced by our E.coli expression system and the target gene encoding Met1-Ser456 is expressed with a 6His tag at the C-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product BLNK Protein
-
B-Cell Linker (BLNK) (AA 1-456) protein (GST tag)
BLNK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 301-400) protein (GST tag)BLNK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 1-456) protein (His tag)BLNK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
B-Cell Linker (BLNK) (AA 1-456) protein (Strep Tag)Crystallography grade BLNK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
B-Cell Linker (BLNK) (AA 1-457) protein (Strep Tag)Crystallography grade BLNK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- B-Cell Linker (BLNK)
- Alternative Name
- BLNK/Ly-57 (BLNK Products)
- Synonyms
- BLNK Protein, blnk Protein, MGC147045 Protein, BASH Protein, Bca Protein, Ly-57 Protein, Ly57 Protein, Lyw-57 Protein, SLP-65 Protein, AGM4 Protein, BLNK-S Protein, LY57 Protein, SLP65 Protein, bca Protein, B-cell linker Protein, B cell linker Protein, BLNK Protein, blnk Protein, Blnk Protein
- Background
-
Background: B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.
Synonym: B-Cell Linker Protein, B-Cell Adapter Containing a SH2 Domain Protein, B-Cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa, SLP-65, BLNK, BASH, SLP65
- Molecular Weight
- 51.5 kDa
- UniProt
- Q8WV28
- Pathways
- BCR Signaling
-
