B-Cell Linker Protein (BLNK) (AA 1-456) (His tag)

Catalog No. ABIN7319686

This Recombinant B-Cell Linker protein is produced in Escherichia coli (E. coli).

Quick Overview for B-Cell Linker Protein (BLNK) (AA 1-456) (His tag) (ABIN7319686)

Target: B-Cell Linker (BLNK)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-456

Purification tag / Conjugate: This B-Cell Linker protein is labelled with His tag.

Purpose: Recombinant Human BLNK/Ly-57 Protein (His Tag)

Sequence: Met1-Ser456

Characteristics: Recombinant Human BLNK is produced by our E.coli expression system and the target gene encoding Met1-Ser456 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK/Ly-57

Background: B-Cell Adapter Containing a SH2 Domain Protein,B-Cell Adapter Containing a Src Homology 2 Domain Protein,B-Cell Linker Protein,BASH,BLNK,Cytoplasmic Adapter Protein,SLP-65,SLP65,Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa,B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.

Molecular Weight:

Calculated MW: 51.5 kDa

Observed MW: 40-80 kDa

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling