BCAS2 Protein (T7 tag,His tag)

Catalog No. ABIN7318195

This Recombinant BCAS2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for BCAS2 Protein (T7 tag,His tag) (ABIN7318195)

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This BCAS2 protein is labelled with T7 tag,His tag.

Purpose: Recombinant Human BCAS2/DAM1 Protein (His & T7 Tag)

Sequence: Ala2-Phe225

Characteristics: Recombinant Human Breast Carcinoma-Amplified Sequence 2 is produced by our E.coli expression system and the target gene encoding Ala2-Phe225 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 2 mM DTT, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Alternative Name: BCAS2/DAM1

Background:

Background: Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.

Synonym: Pre-mRNA-Splicing Factor SPF27, Breast Carcinoma-Amplified Sequence 2, DNA Amplified in Mammary Carcinoma 1 Protein, Spliceosome-Associated Protein SPF 27, BCAS2, DAM1

Molecular Weight: 28.6 kDa

UniProt: O75934