BCAS2 Protein (AA 2-225) (His tag,T7 tag)
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- Target See all BCAS2 Proteins
- BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-225
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BCAS2 protein is labelled with His tag,T7 tag.
- Purpose
- Recombinant Human BCAS2/DAM1 (C-6His, N-T7 tag)
- Sequence
- MASMTGGQQM GRGSMAGTGL VAGEVVVDAL PYFDQGYEAP GVREAAAALV EEETRRYRPT KNYLSYLTAP DYSAFETDIM RNEFERLAAR QPIELLSMKR YELPAPSSGQ KNDITAWQEC VNNSMAQLEH QAVRIENLEL MSQHGCNAWK VYNENLVHMI EHAQKELQKL RKHIQDLNWQ RKNMQLTAGS KLREMESNWV SLVSKNYEIE RTIVQLENEI YQIKQQHGEA NKENIRQDFL EHHHHHH
- Characteristics
- Recombinant Human Breast Carcinoma-Amplified Sequence 2/BCAS2 is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Phe225) of Human BCAS2 fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BCAS2 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 2 mM DTT, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))
- Alternative Name
- Breast Carcinoma-Amplified Sequence 2/BCAS2 (BCAS2 Products)
- Synonyms
- DAM1 Protein, SPF27 Protein, Snt309 Protein, 6430539P16Rik Protein, AI132645 Protein, C76366 Protein, C80030 Protein, BCAS2 Protein, cb302 Protein, zgc:101730 Protein, BCAS2, pre-mRNA processing factor Protein, breast carcinoma amplified sequence 2 Protein, breast carcinoma amplified sequence 2 L homeolog Protein, BCAS2 Protein, Bcas2 Protein, bcas2 Protein, bcas2.L Protein, CpipJ_CPIJ003889 Protein
- Sub Type
- Fusionprotein
- Background
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Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.
Alternative Names: Pre-mRNA-Splicing Factor SPF27, Breast Carcinoma-Amplified Sequence 2, DNA Amplified in Mammary Carcinoma 1 Protein, Spliceosome-Associated Protein SPF 27, BCAS2, DAM1 - Molecular Weight
- 28.58 kDa
- UniProt
- O75934
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