Butyrylcholinesterase Protein (BCHE) (His tag)
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- Target See all Butyrylcholinesterase (BCHE) Proteins
- Butyrylcholinesterase (BCHE)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Butyrylcholinesterase protein is labelled with His tag.
- Purpose
- Recombinant Mouse Butyrylcholinesterase/BCHE Protein (His Tag)(Active)
- Sequence
- Met 1-Leu 603
- Characteristics
- A DNA sequence encoding the mouse BCHE (NP_033868.3) (Met 1-Leu 603) was expressed, with a C-terminal polyhistidine tag.
- Purity
- > 98 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 70 nmol/min/μg.
- Top Product
- Discover our top product BCHE Protein
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Butyrylcholinesterase (BCHE) (AA 493-602) protein (GST tag)
BCHE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Butyrylcholinesterase (BCHE) (AA 29-602) protein (His tag)Crystallography grade BCHE Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Butyrylcholinesterase (BCHE) (AA 30-603) protein (His tag)Crystallography grade BCHE Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Butyrylcholinesterase (BCHE) (AA 1-141), (full length) protein (His tag)BCHE Origin: Dog Host: Yeast Recombinant > 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 25 mM Tris, 100 mM NaCl, pH 7.5
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Butyrylcholinesterase (BCHE)
- Alternative Name
- Butyrylcholinesterase/BCHE (BCHE Products)
- Synonyms
- che Protein, che1 Protein, bche Protein, cholinesterase Protein, CHE1 Protein, CHE2 Protein, E1 Protein, C730038G20Rik Protein, butyrylcholinesterase L homeolog Protein, butyrylcholinesterase Protein, bche.L Protein, bche Protein, BCHE Protein, Bche Protein
- Background
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Background: Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.
Synonym: C730038G20Rik
- Molecular Weight
- 66.7 kDa
- NCBI Accession
- NP_033868
- Pathways
- Peptide Hormone Metabolism
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