C-Type Lectin Domain Family 6, Member A (CLEC6A) (Active) protein (His tag)

Catalog No. ABIN7320341

Product Details

Target: C-Type Lectin Domain Family 6, Member A (CLEC6A)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse CLEC6A/Dectin-2 Protein (His Tag)(Active)

Sequence: Ile 44-Leu 209

Characteristics: A DNA sequence encoding the mouse CLEC4N (NP_064385.1) extracellular domain (Ile 44-Leu 209) was expressed, with a C-terminal polyhistidine tag.

Purity: > 97 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to agglutinate human red blood cells. Mouse CLEC4N at 25 μg/ml could agglutinate 1 % HRBC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: C-Type Lectin Domain Family 6, Member A (CLEC6A)

Alternative Name: CLEC6A/Dectin-2 (CLEC6A Products)

Synonyms: CLEC4N Protein, CLECSF10 Protein, CLEC6A Protein, C-type lectin domain containing 6A Protein, C-type lectin domain family 6, member A Protein, CLEC6A Protein, Clec6a Protein

Background:

Background: C-type lectin domain family 4 member N (CLEC4N), also known as Dectin-2, is a C-type lectin expressed by dendritic cells (DCs) and macrophages. Members of the C-type lectin domain (CTLD) superfamily are metazoan proteins functionally important in glycoprotein metabolism, mechanisms of multicellular integration and immunity. They share a common fold and are involved in a variety of functions, such as generalized defense mechanisms against foreign agents, discrimination between healthy and pathogen-infected cells, and endocytosis and blood coagulation. Genome-level studies on human, elegans and melanogaster demonstrated almost complete divergence among invertebrate and mammalian families of CTLD-containing proteins (CTLDcps). The vertebrate CTLDcp family was essentially formed early in vertebrate evolution and is completely different from the invertebrate families. The composition of the CTLDcp superfamily in fish and mammals suggests that large scale duplication events played an important role in the evolution of vertebrates. Dectin-2 is important in host defense against C. albicans by inducing Th17 cell differentiation. Dectin-2 constitutes a major fungal pattern recognition receptor (PRR) that can couple to the Syk-CARD9 innate signaling pathway to activate DCs and regulate adaptive immune responses to fungal infection.

Synonym: Clec4n,Clecsf10,Nkcl

Molecular Weight: 20.9 kDa

NCBI Accession: NP_064385