CALML5 Protein (His-GST)

Catalog No. ABIN7317487

Recombinant CALML5 protein expressed in Escherichia coli (E. coli).

Quick Overview for CALML5 Protein (His-GST) (ABIN7317487)

Target: CALML5 (Calmodulin-Like 5 (CALML5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CALML5 protein is labelled with His-GST.

Purpose: Recombinant Human CALML5/CLSP Protein (His & GST Tag)

Sequence: Met 1-Glu 146

Characteristics: A DNA sequence encoding the human CALML5 (AAH39172.1) (Met 1-Glu 146) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 1 mM DTT, 0.5 mM GSH, 10 % glycerol, pH 7.8
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CALML5 (Calmodulin-Like 5 (CALML5))

Alternative Name: CALML5/CLSP

Background: CLSP,Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.

Molecular Weight:

Calculated MW: 44.2 kDa

Observed MW: 43 kDa

Gene ID: 51806

UniProt: Q9NZT1

Pathways: Phototransduction