CA5A Protein (His tag)

Catalog No. ABIN7194574

This Recombinant CA5A protein is expressed in Escherichia coli (E. coli).

Quick Overview for CA5A Protein (His tag) (ABIN7194574)

Target: CA5A (Carbonic Anhydrase VA (CA5A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 96 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CA5A protein is labelled with His tag.

Purpose: Recombinant Human CA5A/CA-VA Protein (His Tag)

Sequence: Ala 40-Ser 305

Characteristics: A DNA sequence encoding the mature form of human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured by its esterase activity. The specific activity is > 500 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM NaAc, 50 mM NaCl, 0.05 % Brij 35, pH 5.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CA5A (Carbonic Anhydrase VA (CA5A))

Alternative Name: CA5A/CA-VA

Background: CA5,CA5AD,CA5D,CAV,CAVA,Carbonic Anhydrase VA,GS1-21A4.1,Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

Molecular Weight:

Calculated MW: 31.6 kDa

Observed MW: 33 kDa

Gene ID: 763

NCBI Accession: NP_001730

UniProt: P35218