Carboxylesterase 2E (CES2E) (Active) protein (His tag)

Catalog No. ABIN7194593

Recombinant Carboxylesterase 2E protein expressed in HEK-293 Cells.

Quick Overview for Carboxylesterase 2E (CES2E) (Active) protein (His tag) (ABIN7194593)

Target: Carboxylesterase 2E (CES2E)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 88 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse Carboxylesterase 2E/CES2E Protein (His Tag) (Active)

Sequence: Met 1-His 556

Characteristics: A DNA sequence encoding the extracellular domain of mouse CES5 (NP_766347.1) (Met 1-His 556) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Carboxylesterase 2E (CES2E)

Alternative Name: Carboxylesterase 2E/CES2E

Background:

Background: Carboxylesterase belongs to the class of serine hydrolases family which includes Chymotrypsin and Acetylcholinesterase. Carboxylesterase is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Carboxylesterase is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. Carboxylesterase 5 (CES5), also known as cauxin or CES7, is one of CES enzyme families exerting role in catalyzing hydrolytic and transesterfication reactions with broad substrat specifity. CES5 is reported in high concentrations in the urine of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for carboxylesterase 5 may include regulating urinary levels of cat pheromone, catalyzing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics.

Synonym: Pyrethroid hydrolase Ces2e, carboxylesterase 2E, Ces5,1700081L16Rik,1700122C07Rik,BB081581,cauxin,Ces7,Gm503

Molecular Weight: 60.6 kDa

NCBI Accession: NP_766347