Carboxylesterase 2E (CES2E) (AA 27-559) (Active) protein (His tag)
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- Target See all Carboxylesterase 2E (CES2E) Proteins
- Carboxylesterase 2E (CES2E)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 27-559
- Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Sequence
- QDSASPIRNT HTGQVRGSLV HVKDTDIAVH TFLGIPFAKP PVGPLRFAPP EAPEPWSGVR DGTSHPNMCL QNDNLMGSED LKMMNLILPP ISMSEDCLYL NIYVPAHAHE GSNLPVMVWI HGGALTVGMA SMYDGSMLAA TEDVVVVAIQ YRLGVLGFFS TGDQHAKGNW GYLDQVAALR WVQQNIVHFG GNPDRVTIFG ESAGGTSVSS HVVSPMSQGL FHGAIMESGV AVLPDLISSS SEMVHRIVAN LSGCAAVNSE TLMCCLRGKN EAEMLAINKV FKIIPGVVDG EFLPKHPQEL MASKDFHPVP SIIGINNDEY GWILPTIMDP AQKIEEITRK TLPAVLKSTA LKMMLPPECG DLLMEEYMGD TEDPETLQAQ FREMKGDFMF VIPALQVAHF QRSHAPVYFY EFQHRPSFFK DFRPPYVKAD HGDEIFLVFG YQFGNIKLPY TEEEEQLSRR IMKYWANFAR HGNPNSEGLP YWPVMDHDEQ YLQLDIQPSV GRALKARRLQ FWTKTLPQKI QELKGSQERH KELLEHHHHH H
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 30 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.
- Top Product
- Discover our top product CES2E Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Carboxylesterase 2E (CES2E)
- Alternative Name
- Pyrethroid hydrolase Ces2e (CES2E Products)
- Synonyms
- 9030624L02Rik Protein, Ces5 Protein, carboxylesterase 2E Protein, Ces2e Protein
- Background
- CES2E, also known as pyrethroid hydrolase Ces2e, is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it is widely distributed in cells especially in mammalian liver. It is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Recombinant mouse CES2E, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 60.5kDa (541aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_766347
- UniProt
- Q8BK48
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