CPA1 Protein (His tag)

Catalog No. ABIN7194598

This Recombinant CPA1 protein is expressed in HEK-293 Cells.

Quick Overview for CPA1 Protein (His tag) (ABIN7194598)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 96 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CPA1 protein is labelled with His tag.

Purpose: Recombinant Mouse Carboxypeptidase A1/CPA1 Protein (His Tag)(Active)

Sequence: Met 1-Tyr 419

Characteristics: A DNA sequence encoding the mouse CPA1 (NP_079626.2) (Met 1-Tyr 419) precusor was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >6,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: Carboxypeptidase A1/CPA1

Background:

Background: Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of  residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Synonym: 0910001L12Rik;Cpa

Molecular Weight: 47 kDa

NCBI Accession: NP_079626