CPB2 Protein (His tag)

Catalog No. ABIN7321061

This Recombinant CPB2 protein is expressed in HEK-293 Cells.

Quick Overview for CPB2 Protein (His tag) (ABIN7321061)

Target: CPB2 (Carboxypeptidase B2 (Plasma) (CPB2))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CPB2 protein is labelled with His tag.

Purpose: Recombinant Rat Carboxypeptidase B2/CPB2 Protein (His Tag)

Sequence: Met1-Ser422

Characteristics: A DNA sequence encoding the rat Cpb2 (NP_446069.1) (Met1-Ser422) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPB2 (Carboxypeptidase B2 (Plasma) (CPB2))

Alternative Name: Carboxypeptidase B2/CPB2

Background:

Background: Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Synonym: CPB2

Molecular Weight: 48 kDa

NCBI Accession: NP_446069

Pathways: Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis