CROT Protein (His tag)

Catalog No. ABIN7317695

This Recombinant CROT protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CROT Protein (His tag) (ABIN7317695)

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 93 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CROT protein is labelled with His tag.

Purpose: Recombinant Human CROT Protein (474 Leu/Val, His Tag)

Sequence: Met 1-Leu 612, 474 Leu/Val

Characteristics: A DNA sequence encoding the human CROT (Q9UKG9) (Met 1-Leu 612, 474 Leu/Val) was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Alternative Name: CROT

Background: COT,Carnitine octanoyltransferase (CROT or COT), also known as octanoyl-CoA: L-carnitine O-octanoyltransferase, medium-chain/long-chain carnitine acyltransferase, and carnitine medium-chain acyltransferase, is a carnitine acyltransferase belonging to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. Carnitine octanoyltransferase (CROT or COT) facilitate the transport of medium- and long-chain fatty acids through the peroxisomal and mitochondrial membranes. It is physiologically inhibited by malonyl-CoA. COT also has functions in efficiently converting one of the end products of the peroxisomal beta-oxidation of pristanic acid, 4, 8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.

Molecular Weight:

Calculated MW: 71.5 kDa

Observed MW: 65 kDa

Gene ID: 54677

UniProt: Q9UKG9

Pathways: Monocarboxylic Acid Catabolic Process