CPT1B Protein (His tag)

Catalog No. ABIN7194637

This Recombinant CPT1B protein is expressed in HEK-293 Cells.

Quick Overview for CPT1B Protein (His tag) (ABIN7194637)

Target: CPT1B (Carnitine Palmitoyltransferase 1B (Muscle) (CPT1B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CPT1B protein is labelled with His tag.

Purpose: Recombinant Mouse MCPT1 Protein (His Tag)

Sequence: Met 1-Lys 246

Characteristics: A DNA sequence encoding the mouse MCPT1 (NP_032596.1) (Met 1-Lys 246) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPT1B (Carnitine Palmitoyltransferase 1B (Muscle) (CPT1B))

Alternative Name: MCPT1

Background:

Background: Mast Cell Protease 1 (MMCP-1), also known as MCP-1, MCPT-1 and β-chymase, is a member of the Chymase family of chymotrypsin-like serine proteases. MCPT-1 is a 26 kDa β-chymase that is a component of mast cell granules. It is a 226 amino acid (aa) protein that has a conserved pattern of six cysteines and one potential glycosylation site. The granule-derived mouse mast cell proteases-1 and -2 (mMCP-1 and -2) colocalize in similar quantities in mucosal mast cells but micrograms of mMCP-1 compared with nanograms of mMCP-2 are detected in peripheral blood during intestinal nematode infection. mMCP-1 isolated from serum is complexed with serpins and both the accumulation and the longevity of mMCP-1 in blood is due to complex formation, protecting it from a pathway that rapidly clears mMCP-2, which is unable to form complexes with serpins. The mucosal mast cell (MMC) granule-specific beta-chymase, mouse mast cell protease-1 (mMCP-1), is released systemically into the bloodstream early in nematode infection before parasite-specific IgE responses develop and TGF-beta1 induces constitutive release of mMCP-1 by homologues of MMC in vitro. Expression of mMCP-1 is largely restricted to intraepithelial MMC and is thought to play a role in the regulation of epithelial permeability. Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C). MCPT-1 is upregulated in the intestine in response to nematode infection, or in systemic mucosa in response to anaphylaxis. Like human α-chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure. The intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1.

Synonym: AV080368;Mcp-1

Molecular Weight: 26.8 kDa

NCBI Accession: NP_032596

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process