CSNK1G1 Protein (His-GST)

Catalog No. ABIN7317070

Recombinant CSNK1G1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CSNK1G1 Protein (His-GST) (ABIN7317070)

Target: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CSNK1G1 protein is labelled with His-GST.

Purpose: Recombinant Human CSNK1G1/CKI-gamma 1 Protein (His & GST Tag)

Sequence: Met 1-Lys 422

Characteristics: A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 4 nmol/min/mg using casein as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Alternative Name: CSNK1G1/CKI-gamma 1

Background: CK1gamma1,Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Thecasein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators ofsignal transductionpathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53 % -98 % identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.

Molecular Weight:

Calculated MW: 76.3 kDa

Observed MW: 66 kDa

Gene ID: 53944

NCBI Accession: NP_071331

UniProt: Q9HCP0

Pathways: Hedgehog Signaling