CD1b Protein (CD1B) (Fc Tag)

Catalog No. ABIN7317475

Recombinant CD1b protein expressed in HEK-293 Cells.

Quick Overview for CD1b Protein (CD1B) (Fc Tag) (ABIN7317475)

Target: CD1b (CD1B)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD1b protein is labelled with Fc Tag.

Purpose: Recombinant Human CD1B/CD1A Protein (Fc Tag)

Sequence: Met 1-Ser 303

Characteristics: A DNA sequence encoding the human CD1B (NP_001755) (Met1-Ser 303) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD1b (CD1B)

Alternative Name: CD1B/CD1A

Background: CD1,CD1A,CD1B,R1,CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Molecular Weight:

Calculated MW: 58.6 kDa

Observed MW: 59-69 kDa

Gene ID: 910

NCBI Accession: NP_001755

UniProt: P29016