CD27 Protein (His tag)

Catalog No. ABIN7194765

This Recombinant CD27 protein is produced in HEK-293 Cells.

Quick Overview for CD27 Protein (His tag) (ABIN7194765)

Target: CD27 (CD27 Molecule (CD27))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 96 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD27 protein is labelled with His tag.

Purpose: Recombinant Mouse CD27/TNFRSF7 Protein (His Tag)

Sequence: Met 1-Arg 182

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27/TNFRSF7

Background: CD27 antigen,CD27L receptor,S152,T-cell activation antigen CD27,Tnfrsf7,Tp55,Tumor necrosis factor receptor superfamily member 7,CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Molecular Weight:

Calculated MW: 19.6 kDa

Observed MW: 35 kDa

Gene ID: 21940

NCBI Accession: NP_001028298

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints