CD27 Protein (Fc-His Tag)
Quick Overview for CD27 Protein (Fc-His Tag) (ABIN7318277)
Target
See all CD27 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CD27 protein is labelled with Fc-His Tag.
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Purpose
- Recombinant Human CD27/TNFRSF7 Protein (Fc & His Tag)
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Sequence
- Thr 21-Ile192
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Characteristics
- Recombinant Human CD27 is produced by our Mammalian expression system and the target gene encoding Thr21-Ile192 is expressed with a Fc, 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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CD27 Molecule (CD27) (AA 20-191) protein (Fc Tag)
CD27 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD27 Molecule (CD27) (AA 20-191) protein (His tag,mFc Tag)CD27 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD27 Molecule (CD27) (AA 21-192) (Active) protein (His tag)CD27 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD27 Molecule (CD27) (AA 20-191) (Active) protein (hFc Tag)CD27 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD27 Molecule (CD27) (AA 21-192) (Active) protein (His tag)CD27 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD27 Molecule (CD27) (AA 24-182) (Active) protein (mFc Tag)CD27 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD27 Molecule (CD27) (AA 1-260) protein (GST tag)CD27 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
CD27 Molecule (CD27) (AA 21-182) (Active) protein (Fc Tag)CD27 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD27 Molecule (CD27) (AA 24-182) protein (Fc Tag)CD27 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD27 Molecule (CD27) (AA 21-191) (Active) protein (hIgG-His-tag)CD27 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS, AcA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- CD27 (CD27 Molecule (CD27))
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Alternative Name
- CD27/TNFRSF7
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Background
- S152,S152. LPFS2,S152.LPFS2,T14,TNFRSF7,Tp55,CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.
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Molecular Weight
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Calculated MW: 47.2 kDa
Observed MW: 60 kDa
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Gene ID
- 939
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UniProt
- P26842
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Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Target
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