PD-L1 Protein (His tag)

Catalog No. ABIN7319492

This Recombinant PD-L1 protein is expressed in Human Cells.

Quick Overview for PD-L1 Protein (His tag) (ABIN7319492)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PD-L1 protein is labelled with His tag.

Purpose: Recombinant Human PD-L1/B7-H1/CD274 Protein (His Tag)(Active)

Sequence: Phe19-Thr239

Characteristics: Recombinant Human PD-L1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human PD-1-Fc(Cat: PKSM041289) at 10μg/ml(100 μl/well) can bind Human PD-L1-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 % Trehalose, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1/B7-H1/CD274

Background:

Background: CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Synonym: Programmed Cell Death 1 Ligand 1, PD-L1, PDCD1 Ligand 1, Programmed Death Ligand 1, B7 Homolog 1, B7-H1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1

Molecular Weight: 26.33 kDa

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints