Quick Overview for PD-L1 Protein (His tag) (ABIN7194779)
Target
See all PD-L1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PD-L1 protein is labelled with His tag.
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Purpose
- Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)
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Sequence
- Met 1-Thr 238
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Characteristics
- A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized recombinant mouse PD1-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6. 25-400 ng/ml.
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CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His-Avi Tag)
PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) protein (His tag,mFc Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (mFc Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (hFc Tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag,AVI tag,Biotin)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD274 (PD-L1) (AA 18-238) protein (His-DYKDDDDK-Strep II Tag)PD-L1 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD274 (PD-L1) (AA 19-238) (Active) protein (Fc Tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- PD-L1 (CD274 (PD-L1))
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Alternative Name
- PD-L1/B7-H1/CD274
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Background
- A530045L16Rik Protein,B7h1 Protein,Mouse,Pdcd1l1 Protein,Pdcd1lg1 Protein,Pdl1 Protein,Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
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Molecular Weight
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Calculated MW: 26.3 kDa
Observed MW: 40-45 kDa
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NCBI Accession
- NP_068693
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UniProt
- Q3U472
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Pathways
- Cancer Immune Checkpoints
Target
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