DCL1 Protein (AA 1-165) (Fc Tag)

Catalog No. ABIN7321088

This Recombinant DCL1 protein is produced in HEK-293 Cells.

Quick Overview for DCL1 Protein (AA 1-165) (Fc Tag) (ABIN7321088)

Target: DCL1 (CD302) (CD302)

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-165

Purification tag / Conjugate: This DCL1 protein is labelled with Fc Tag.

Purpose: Recombinant Rat CD302/CLEC13A Protein (Fc Tag)

Sequence: Met1-His165

Characteristics: A DNA sequence encoding the rat CD302 (Met1-His165) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: DCL1 (CD302) (CD302)

Alternative Name: CD302/CLEC13A

Background: CD302,CD302/CLEC13A (C-type lectin domain family 13 member A), also known as C-type lectin receptor DCL-1, is a type I transmembrane C-type lectin DCL-1/CD302. DCL-1 protein was highly conserved among the human, mouse, and rat orthologs. DCL-1 ectodomain contains only one CRD, whereas other type I transmembrane C-type lectins contain more than one domain (e.g. selectins and MMR). DCL-1 CP contains several putative motifs, including a Tyr-based internalization, a cluster of acidic amino acids, and Ser and Tyr phosphorylation motifs, suggesting that DCL-1 CP mediates not only endocytosis and late endosome targeting but also signaling. DCL-1 may be another cell/matrix adhesion receptor integrated in cell adhesion complexes and that DCL-1 dysfunction may affect APC adhesion and migration, causing suppression of APC function.

Molecular Weight:

Calculated MW: 43.7 kDa

Observed MW: 47 kDa

Gene ID: 295629

NCBI Accession: NP_001013938

UniProt: Q5FVR3

Pathways: Regulation of Actin Filament Polymerization