CD34 Protein (CD34) (His tag)
Quick Overview for CD34 Protein (CD34) (His tag) (ABIN7320223)
Target
See all CD34 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CD34 protein is labelled with His tag.
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Purpose
- Recombinant Mouse CD34 Protein (His Tag)
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Sequence
- Met 1-Thr 287
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Characteristics
- A DNA sequence encoding the extracellular domain of mouse CD34 (NP_001104529.1) (Met 1-Thr 287) was expressed, with a polyhistidine tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 104 cells/well are added to mouse CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately > 30 % will adhere after one hour at 37 °C.
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CD34 (CD34) (AA 32-290) protein (His tag)
CD34 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD34 (CD34) (AA 35-287) (Active) protein (His tag)CD34 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD34 (CD34) (AA 35-287) protein (Fc Tag)CD34 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD34 (CD34) (AA 124-225) protein (His tag)CD34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-290) protein (His-IF2DI Tag)CD34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-290) protein (His tag)CD34 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS
CD34 (CD34) (AA 1-291) protein (Fc Tag)CD34 Origin: Rat Host: HEK-293 Cells Recombinant > 80 % as determined by reducing SDS-PAGE.
CD34 (CD34) (AA 32-141) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
CD34 (CD34) (AA 1-328) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- CD34
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Alternative Name
- CD34
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Background
- AU040960,Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
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Molecular Weight
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Calculated MW: 28.6 kDa
Observed MW: 50-60 kDa
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NCBI Accession
- NP_001104529
Target
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