CD47 Protein (CD47) (His tag)

Catalog No. ABIN7321142

This Recombinant CD47 protein is produced in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (His tag) (ABIN7321142)

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CD47 protein is labelled with His tag.

Purpose: Recombinant Rat CD47 Protein (His Tag)(Active)

Sequence: Met1-Lys140

Characteristics: A DNA sequence encoding the rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD47

Alternative Name: CD47

Background:

Background: CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CD47

Molecular Weight: 15.2 kDa