Quick Overview for CD47 Protein (CD47) (ABIN7194877)
Target
See all CD47 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purpose
- Recombinant Human CD47 Protein
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Sequence
- Met 1-Pro 139
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Characteristics
- A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with six amino acids (LEVLFQ) at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to bind human SIRPA-His in functional ELISA. Measured by its ability to bind mouse SIRPA-His(Cat: PKSM040419)in functional ELISA.
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CD47 (CD47) (AA 19-141) protein (His tag,mFc Tag)
CD47 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD47 (CD47) (AA 19-141) (Active) protein (His tag)CD47 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD47 (CD47) (AA 19-139) (Active) protein (Biotin,His-Avi Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD47 (CD47) (AA 19-140) (Active) protein (His tag)CD47 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag,AVI tag,Biotin)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) protein (DYKDDDDK Tag)CD47 Origin: Human Host: HEK-293 Cells Synthetic Nanodisc ELISA, Imm, SPR, PhD, cryo-EM
CD47 (CD47) (AA 19-141) (Active) protein (His tag)CD47 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag,AVI tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- CD47
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Alternative Name
- CD47
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Background
- IAP,MER6,OA3,CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
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Molecular Weight
- 14.5 kDa
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Gene ID
- 961
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NCBI Accession
- NP_942088
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UniProt
- Q08722
Target
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