CD47 Protein (CD47) (His tag)
Quick Overview for CD47 Protein (CD47) (His tag) (ABIN7319534)
Target
See all CD47 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This CD47 protein is labelled with His tag.
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Purpose
- Recombinant Human CD47/IAP Protein (His Tag)(Active)
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Sequence
- Gln19-Pro139
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Characteristics
- Recombinant Human CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human SIRPA-Fc(Cat: PKSH033061) at 10μg/ml(100 μl/well) can bind Human CD47-His. The ED50 of Human CD47-His is 4.18 ng/ml .
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CD47 (CD47) (AA 19-139) protein (Fc Tag)
CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) protein (His tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (His tag)CD47 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (mFc-His Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD47 (CD47) (AA 19-139) protein (His-Avi Tag,Biotin)CD47 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (His tag)CD47 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (Fc Tag)CD47 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag,AVI tag,Biotin)CD47 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate, 150 mM NaCl, pH 8.0.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD47
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Alternative Name
- CD47/IAP
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Background
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Background: CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Synonym: Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, IAP, Protein MER6, CD47, MER6
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Molecular Weight
- 14.76 kDa
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UniProt
- Q08722
Target
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