CD48 Protein (CD48) (His tag)

Catalog No. ABIN7320284

This Recombinant CD48 protein is produced in HEK-293 Cells.

Quick Overview for CD48 Protein (CD48) (His tag) (ABIN7320284)

Target: CD48

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD48 protein is labelled with His tag.

Purpose: Recombinant Mouse CD48 Protein (His Tag)

Sequence: Met 1-Arg 216

Characteristics: A DNA sequence encoding the mouse CD48 (NP_031675.1) without the propeptide, corresponding to the amino acids (Met 1-Arg 216) was expressed with a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to bind human 2B4 and biotinylated recombinant mouse CD2 in functional ELISA.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD48

Alternative Name: CD48

Background: AI449234,AW610730,BCM1,BLAST-1,BLAST1,Bcm-1,CD48,MEM-102,SLAM Family Member 2,SLAMF2,Sgp-60,Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Molecular Weight: 23.7 kDa

Gene ID: 12506

NCBI Accession: NP_031675

UniProt: P18181