CD6 Protein (CD6) (Fc Tag)

Catalog No. ABIN7317120

Recombinant CD6 protein expressed in HEK-293 Cells.

Quick Overview for CD6 Protein (CD6) (Fc Tag) (ABIN7317120)

Target: CD6

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD6 protein is labelled with Fc Tag.

Purpose: Recombinant Human CD6/TP120 (Fc Tag)(Active)

Sequence: Met 1-Glu398

Characteristics: A DNA sequence encoding the human CD6 (NP_006716.3) (Met1-Glu398) was expressed with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8x10E4 cells/well are added to CD6-Fc coated plates (10 μg/mL, 100 μL/well), approximately >70% will adhere after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD6

Alternative Name: CD6/TP120

Background:

Background: T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Synonym: TP120

Molecular Weight: 67.3 kDa

NCBI Accession: NP_006716