CD72 Protein (AA 117-226) (His-TRX)

Catalog No. ABIN7318196

This Recombinant CD72 protein is produced in Escherichia coli (E. coli).

Quick Overview for CD72 Protein (AA 117-226) (His-TRX) (ABIN7318196)

Target: CD72 (CD72 Molecule (CD72))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 117-226

Purification tag / Conjugate: This CD72 protein is labelled with His-TRX.

Purpose: Recombinant Human CD72/Lyb-2 Protein (Trx&His)

Sequence: Arg117-Cys226

Characteristics: Recombinant Human B-Cell Differentiation Antigen CD72 is produced by our E.coli expression system and the target gene encoding Arg117-Cys226 is expressed with a Trx, 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72/Lyb-2

Background: B-Cell Differentiation Antigen CD72,CD72,Lyb-2,B-Cell Differentiation Antigen CD72 (CD72) is a single-pass type II membrane protein. CD72 exists as a disulfide-linked homodimer and contains one C-type lectin domain. CD72 is expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells. CD72 is a ligand for CD5. CD72 associates with CD5, interacts with PTPN6/SHP-1 and plays a role in B-cell proliferation and differentiation. CD72 associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP-1.

Molecular Weight:

Calculated MW: 30.6 kDa

Observed MW: 26-35 kDa

Gene ID: 971

UniProt: P21854

Pathways: BCR Signaling