CD86 Protein (CD86) (His tag)

Catalog No. ABIN7194975

This Recombinant CD86 protein is produced in HEK-293 Cells.

Quick Overview for CD86 Protein (CD86) (His tag) (ABIN7194975)

Target: CD86

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD86 protein is labelled with His tag.

Purpose: Recombinant Mouse CD86/B7-2 Protein (His Tag)

Sequence: Met 1-Glu 245

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6. 4-800 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD86

Alternative Name: CD86/B7-2

Background: Activation B7-2 antigen,B7,B7-2,B7.2,B70,CD86,CLS1,Cd28l2,ETC-1,Early T-cell costimulatory molecule 1,Ly-58,Ly58,MB7,MB7-2,T-lymphocyte activation antigen CD86,TS/A-2,CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Molecular Weight:

Calculated MW: 26.8 kDa

Observed MW: 40-55 kDa

Gene ID: 12524

NCBI Accession: NP_062261

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation