CD8 alpha Protein (Fc Tag)

Catalog No. ABIN7321149

This Recombinant CD8 alpha protein is expressed in HEK-293 Cells.

Quick Overview for CD8 alpha Protein (Fc Tag) (ABIN7321149)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CD8 alpha protein is labelled with Fc Tag.

Purpose: Recombinant Rat CD8A/MAL Protein (Fc Tag)

Sequence: Met1-Tyr189

Characteristics: A DNA sequence encoding the rat CD8A (P07725) (Met1-Tyr189) was expressed with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A/MAL

Background:

Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I  membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Synonym: CD8A

Molecular Weight: 45 kDa

UniProt: P07725

Pathways: TCR Signaling