CD8 alpha Protein (AA 22-182) (His-Avi Tag,Biotin)

Catalog No. ABIN7274259

Product Details

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Protein Type: Recombinant

Protein Characteristics: AA 22-182

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD8 alpha protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human CD8 alpha/CD8A Protein

Sequence: Ser22-Asp182

Characteristics: Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Asp182.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD8 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha (CD8A Products)

Synonyms: cd8 Protein, LOC100136450 Protein, BB154331 Protein, Ly-2 Protein, Ly-35 Protein, Ly-B Protein, Lyt-2 Protein, zgc:136643 Protein, CD8 Protein, Leu2 Protein, MAL Protein, p32 Protein, RHACD8-4 Protein, CD8a molecule Protein, uncharacterized LOC100125537 Protein, CD8 alpha Protein, CD8 antigen, alpha chain Protein, T-cell surface glycoprotein CD8 alpha chain Protein, Cd8a Protein, LOC100125537 Protein, LOC100136450 Protein, CD8A Protein, cd8a Protein, LOC100126556 Protein

Background: CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.

Molecular Weight: 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

Pathways: TCR Signaling