CDC42BPB Protein (His-GST)

Catalog No. ABIN7317544

This Recombinant CDC42BPB protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CDC42BPB Protein (His-GST) (ABIN7317544)

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CDC42BPB protein is labelled with His-GST.

Purpose: Recombinant Human CDC42BPB Protein (His & GST Tag)

Sequence: Met 1-His 427

Characteristics: A DNA sequence encoding the amino acid (Met 1-His 427) of human CDC42BPB (Q9Y5S2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM PBS, 500 mM NaCl, 10 % glycerol, 2 mM GSH, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Alternative Name: CDC42BPB

Background: MRCKB,CDC42BPB is a member of the serine / threonine protein kinase family that contains a Cdc42 / Rsc-binding p21 binding domain similar to that of PAK kinase. The kinase domain of this protein is related to the myotonic dystrophy kinase related ROK and this kinase may have functions in downstream regulating of Cdc42 in cytoskeletal recognization. It has been reported that the CDC42BPB protein take part in regulating numerous cellular functions by binding to members of a serine / threonine protein kinase subfamily. These functions include the remodeling of the cell cytoskeleton that is a feature of cell growth and differentiation.

Molecular Weight:

Calculated MW: 82.4 kDa

Observed MW: 70 kDa

Gene ID: 9578

UniProt: Q9Y5S2