CNTF Receptor alpha Protein (His tag)

Catalog No. ABIN7321238

This Recombinant CNTF Receptor alpha protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CNTF Receptor alpha Protein (His tag) (ABIN7321238)

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Baculovirus infected Insect Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CNTF Receptor alpha protein is labelled with His tag.

Purpose: Recombinant Rat CNTFR/CNTFR-alpha Protein (His Tag)(Active)

Sequence: Met 1-Pro 346

Characteristics: A DNA sequence encoding the rat CNTFR (Q08406-1) (Met 1-Pro 346) was fused with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Rat CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 1.28-160 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Alternative Name: CNTFR/CNTFR-alpha

Background:

Background: Ciliary neurotrophic factor(CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor(CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracelluar CNTF binding subunit(CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF is also a survival factor for neurons of the peripheral sensory sympathetic, and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.

Synonym: Cntfr

Molecular Weight: 39.6 kDa

Pathways: JAK-STAT Signaling, Feeding Behaviour