CNTF Receptor alpha Protein (His tag)

Catalog No. ABIN7534348

Product Details

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CNTF Receptor alpha protein is labelled with His tag.

Purpose: Active Recombinant Human CNTFR-alpha Protein

Sequence: QRHSPQEAPH VQYERLGSDV TLPCGTANWD AAVTWRVNGT DLAPDLLNGS QLVLHGLELG HSGLYACFHR DSWHLRHQVL LHVGLPPREP VLSCRSNTYP KGFYCSWHLP TPTYIPNTFN VTVLHGSKIM VCEKDPALKN RCHIRYMHLF STIKYKVSIS VSNALGHNAT AITFDEFTIV KPDPPENVVA RPVPSNPRRL EVTWQTPSTW PDPESFPLKF FLRYRPLILD QWQHVELSDG TAHTITDAYA GKEYIIQVAA KDNEIGTWSD WSVAAHATPW TEEPRHLTTE AQAAETTTST TSSLAPPPTT KICDPGELGS GGGP

Specificity: Gln23-Pro346

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CNTFR at 3 μg/mL (100 μL/well) can bind Recombinant Human CNTF with a linear range of 0.078-6.3 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Alternative Name: CNTFR-alpha (CNTFR Products)

Synonyms: CNTFR-alpha Protein, CNTFRALPHA Protein, Cntfralpha Protein, cb549 Protein, fj33e02 Protein, sb:cb549 Protein, wu:fj33e02 Protein, si:bz76a6.1 Protein, si:dz248f2.1 Protein, si:rp71-76a6.1 Protein, ciliary neurotrophic factor receptor Protein, ciliary neurotrophic factor receptor L homeolog Protein, CNTFR Protein, cntfr.L Protein, Cntfr Protein, cntfr Protein

Background:

Description: CNTF is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi- subunit receptor complex, consisting of an extracelluar CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia.

Name: CNTFR

Gene ID: 1271

UniProt: P26992

Pathways: JAK-STAT Signaling, Feeding Behaviour