CLK3 Protein (CLK3) (GST tag)

Catalog No. ABIN7317780

This Recombinant CLK3 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CLK3 Protein (CLK3) (GST tag) (ABIN7317780)

Target: CLK3

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CLK3 protein is labelled with GST tag.

Purpose: Recombinant Human CLK3 Protein (GST Tag)

Sequence: Met 1-Arg 490

Characteristics: A DNA sequence encoding the human CLK3 isoform b (NP_003983.2) (Met 1-Arg 490) was fused with the GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM GSH, 0.5 mM PMSF, 25 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CLK3

Alternative Name: CLK3

Background: PHCLK3,PHCLK3/152,Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2(T) and hCLK3(T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.

Molecular Weight:

Calculated MW: 85.0 kDa

Observed MW: 70 kDa

Gene ID: 1198

NCBI Accession: NP_003983

UniProt: P49761