CNDP2 Protein (His tag)

Catalog No. ABIN7320351

Recombinant CNDP2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CNDP2 Protein (His tag) (ABIN7320351)

Target: CNDP2 (CNDP Dipeptidase 2 (Metallopeptidase M20 Family) (CNDP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CNDP2 protein is labelled with His tag.

Purpose: Recombinant Mouse CNDP2/CPGL/PEPA Protein (His Tag)(Active)

Sequence: Met 1-Asn 475

Characteristics: A DNA sequence encoding the mouse CNDP2 (NP_075638.2) (Met 1-Asn 475) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave carnosine (β-Ala-His) in a two step assay (mouse CNDP2 concentration 10 μg/ml). The specific activity is >40 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CNDP2 (CNDP Dipeptidase 2 (Metallopeptidase M20 Family) (CNDP2))

Alternative Name: CNDP2/CPGL/PEPA

Background:

Background: Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to the peptidase M20A family. CNDP2 / CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 / CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. It may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform 1 of CNDP2 / CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 of CNDP2 / CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 / CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.

Synonym: CNDP2;CNDP dipeptidase 2;Cytosolic non-specific dipeptidase;Glutamate carboxypeptidase-like protein 1;Dip-2;Pep-1;Pep1

Molecular Weight: 54 kDa

NCBI Accession: NP_075638