CD131 Protein (His tag)

Catalog No. ABIN7317843

This Recombinant CD131 protein is expressed in HEK-293 Cells.

Quick Overview for CD131 Protein (His tag) (ABIN7317843)

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD131 protein is labelled with His tag.

Purpose: Recombinant Human CD131/CSF2RB Protein (His Tag)(Active)

Sequence: Met 1-Trp 443

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Trp 443) of human CSF2RB (NP_000386.1) expressed, fused with a polyhistidine-tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized human CD131 at 10 μg/ml (100 μl/well) can bind biotinylated human EPOR/Fc with a linear range of 0.16-4 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Alternative Name: CD131/CSF2RB

Background:

Background: Colony stimulating factor 2 receptor, beta, low-affinity (CSF2RB) also known as CD131 antigen (CD131), cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta-chain, interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (IL3RB), is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this protein have been reported to be associated with protein alveolar proteinosis (PAP). CD131 belongs to the type I cytokine receptor family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Defects in CD131/CSF2RB are the cause of pulmonary surfactant metabolism dysfunction type 5 (SMDP5). SMDP5 is a rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.

Synonym: CD131,CDw131,IL3RB,IL5RB,SMDP5

Molecular Weight: 50 kDa

NCBI Accession: NP_000386

Pathways: JAK-STAT Signaling