CPLX2 Protein (His tag)

Catalog No. ABIN7317462

Recombinant CPLX2 protein expressed in Escherichia coli (E. coli).

Quick Overview for CPLX2 Protein (His tag) (ABIN7317462)

Target: CPLX2 (Complexin 2 (CPLX2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CPLX2 protein is labelled with His tag.

Purpose: Recombinant Human Complexin-2/CPLX2 Protein (His Tag)

Sequence: Asp 2-Lys 134

Characteristics: A DNA sequence encoding the human CPLX2 (Q6PUV4-1) (Asp 2-Lys 134) was expressed, with a polyhistide tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CPLX2 (Complexin 2 (CPLX2))

Alternative Name: Complexin-2/CPLX2

Background: 921-L,CPX-2,CPX2,Hfb1,Complexin-2 (CPLX2), a member of the complexin/synaphin family, is a soluble pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals. Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Complexins are unable to bind to monomeric SNARE proteins but bind with high affinity to ternary SNARE complexes and with lower affinity to target SNARE complexes. Complexin 1 (CX1) and complexin 2 (CX2) are presynaptic proteins that modulate neurotransmitter release and are used as markers of inhibitory and excitatory synapses, respectively. CPLX2 is localized in pre-synaptic terminals in mature brain. The G71-P89 region of CPLX2 is essential and sufficient for preferential axonal distribution. CPLX2 participates in the Ca(2+)-sensitive regulatory pathway for zymogen granule exocytosis. Complexin-2 is a key player in normal neurological function, and its downregulation could lead to changes in neurotransmitter release sufficient to cause significant behavioural abnormalities such as depression. It is involved in synaptogenesis and the modulation of neurotransmitter release.

Molecular Weight:

Calculated MW: 16.8 kDa

Observed MW: 21 kDa

Gene ID: 10814

UniProt: Q6PUV4

Pathways: Synaptic Vesicle Exocytosis