CPNE1 Protein (His tag)
Quick Overview for CPNE1 Protein (His tag) (ABIN7318335)
Target
See all CPNE1 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CPNE1 protein is labelled with His tag.
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Purpose
- Recombinant Human Copine-1/CPNE1 Protein (His Tag)
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Sequence
- Met 1-Ala537
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Characteristics
- Recombinant Human Copine-1 is produced by our E.coli expression system and the target gene encoding Met1-Ala537 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Copine I (CPNE1) (AA 1-536) protein (His tag)
custom-made CPNE1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Copine I (CPNE1) (AA 1-537) protein (His tag)custom-made CPNE1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Copine I (CPNE1) (AA 1-537) protein (His-IF2DI Tag)CPNE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Copine I (CPNE1) (AA 1-537) protein (GST tag)CPNE1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Copine I (CPNE1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CPNE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Copine I (CPNE1) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)CPNE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 50 mM NaCl, 6 % Trehalose, 3 % Glycine, 5 mM Methionine, 0.05 % Tween 80, pH 5.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- CPNE1 (Copine I (CPNE1))
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Alternative Name
- Copine-1/CPNE1
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Background
- CPN1,CPNE1,Copine I,Copine-1,Copine-1(CPNE1) encodes a calcium-dependent protein which belongs to the copine family. CPNE1contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, CPNE1 does not contain a predicted signal sequence or transmembrane domains. CPNE1 may regulate molecular events at the interface of the cell membrane and cytoplasm. CPNE1 has a broad tissue distribution and it may function in membrane trafficking.
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Molecular Weight
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Calculated MW: 62.3 kDa
Observed MW: 58-66 kDa
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Gene ID
- 8904
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UniProt
- Q99829
Target
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