CSTA Protein (AA 2-98) (His tag)
Quick Overview for CSTA Protein (AA 2-98) (His tag) (ABIN7318371)
Target
See all CSTA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 2-98
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Purification tag / Conjugate
- This CSTA protein is labelled with His tag.
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Purpose
- Recombinant Human Cystatin A/CSTA Protein (His Tag)
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Sequence
- Ile2-Phe98
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Characteristics
- Recombinant Human Cystatin A is produced by our E.coli expression system and the target gene encoding Ile2-Phe98 is expressed with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (His tag)
custom-made CSTA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-97) protein (His tag)custom-made CSTA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)CSTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (His-IF2DI Tag)CSTA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cystatin A (Stefin A) (CSTA) (AA 1-98) (Active) protein (His tag)CSTA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- CSTA (Cystatin A (Stefin A) (CSTA))
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Alternative Name
- Cystatin A/CSTA
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Background
- CSTA,Cystatin-A,Cystatin-AS,STF1,STFA,Stefin-A,Human Cystatin A (CSTA) is a member of family 1 of the cystatin superfamily, which is characterized by lacking of disulphide bonds an carbohydrates. Cystatin A is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L. Cystatin A is also implicated in a number of disease states. Due to altered proteolytic state in cancer progression, Cystatin A may play a role in the proteolytic pathways.
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Molecular Weight
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Calculated MW: 11.8 kDa
Observed MW: 14 kDa
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Gene ID
- 1475
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UniProt
- P01040
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Pathways
- Response to Water Deprivation
Target
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