CSTA Protein (His tag)
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- Target See all CSTA Proteins
- CSTA (Cystatin A (Stefin A) (CSTA))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CSTA protein is labelled with His tag.
- Purpose
- Active Recombinant Human Cystatin-A/CSTA Protein
- Sequence
- MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
- Specificity
- Met1-Phe98
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 1.0 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is <0.7 nM.
- Top Product
- Discover our top product CSTA Protein
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)
CSTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- CSTA (Cystatin A (Stefin A) (CSTA))
- Alternative Name
- Cystatin-A/CSTA (CSTA Products)
- Synonyms
- AREI Protein, STF1 Protein, STFA Protein, CST Protein, CSTA Protein, ATCYSA Protein, FL3-27 Protein, T20B5.8 Protein, T20B5_8 Protein, cystatin A Protein, cystatin A Protein, cystatin A1 Protein, cystatin-A Protein, CSTA Protein, Csta1 Protein, Csta Protein, CYSA Protein, LOC100725920 Protein
- Background
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Description: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.
Name: CSTA,AREI,PSS4,STF1,STFA
- Gene ID
- 1475
- UniProt
- P01040
- Pathways
- Response to Water Deprivation
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