CSTA Protein (AA 1-98) (His tag)
CSTA
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
Active
1 image
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- Target See all CSTA Proteins
- CSTA (Cystatin A (Stefin A) (CSTA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-98
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CSTA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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- Discover our top product CSTA Protein
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)
CSTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CSTA (Cystatin A (Stefin A) (CSTA))
- Alternative Name
- CSTA (CSTA Products)
- Synonyms
- AREI Protein, STF1 Protein, STFA Protein, CST Protein, CSTA Protein, ATCYSA Protein, FL3-27 Protein, T20B5.8 Protein, T20B5_8 Protein, cystatin A Protein, cystatin A Protein, cystatin A1 Protein, cystatin-A Protein, CSTA Protein, Csta1 Protein, Csta Protein, CYSA Protein, LOC100725920 Protein
- Background
- CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 13.1 kDa (118aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_005204
- UniProt
- P01040
- Pathways
- Response to Water Deprivation
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