D Amino Acid Oxidase Protein (His tag)
Quick Overview for D Amino Acid Oxidase Protein (His tag) (ABIN7318393)
Target
See all D Amino Acid Oxidase (DAO) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This D Amino Acid Oxidase protein is labelled with His tag.
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Purpose
- Recombinant Human D-Amino-Acid Oxidase/DAO Protein (His Tag)
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Sequence
- Met 1-Leu347
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Characteristics
- Recombinant Human D-Amino-Acid Oxidase is produced by our E.coli expression system and the target gene encoding Met1-Leu347 is expressed with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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D-Amino-Acid Oxidase (DAO) (AA 1-347) protein (His tag)
custom-made DAO Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
D-Amino-Acid Oxidase (DAO) (AA 1-345) protein (His tag)custom-made DAO Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 20 % Glycerol, 1 mM DTT, 2 mM EDTA, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
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Alternative Name
- D-Amino-Acid Oxidase/DAO
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Background
- D-Amino-Acid Oxidase,DAAO,DAMOX,DAO,D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.
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Molecular Weight
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Calculated MW: 41.6 kDa
Observed MW: 40 kDa
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Gene ID
- 1610
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UniProt
- P14920
Target
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