Desmocollin 2 Protein (DSC2) (His tag)

Catalog No. ABIN7317758

Recombinant Desmocollin 2 protein expressed in HEK-293 Cells.

Quick Overview for Desmocollin 2 Protein (DSC2) (His tag) (ABIN7317758)

Target: Desmocollin 2 (DSC2)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 75 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Desmocollin 2 protein is labelled with His tag.

Purpose: Recombinant Human DSC2/Desmocollin-2 Protein (His Tag)

Sequence: Met 1-Arg 684

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Arg 684) of human DSC2 (Q02487-1) was expressed, with a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Desmocollin 2 (DSC2)

Alternative Name: DSC2/Desmocollin-2

Background: ARVD11,CDHF2,DG2,DGII/III,DSC3,DSC2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in DSC2 are associated with arrhythmogenic right ventricular dysplasia-11. DSC2 is Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSC2 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms.

Molecular Weight:

Calculated MW: 75.0 kDa

Observed MW: 85&100 kDa

Gene ID: 1824

UniProt: Q02487