DCLK1 Protein (AA 1-705) (His-GST)

Catalog No. ABIN7317068

Recombinant DCLK1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for DCLK1 Protein (AA 1-705) (His-GST) (ABIN7317068)

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 84 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-705

Purification tag / Conjugate: This DCLK1 protein is labelled with His-GST.

Purpose: Recombinant Human DCAMKL1 Protein (aa 1-705, His & GST Tag)

Sequence: Met 1-Val 705

Characteristics: A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 6. 1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM PMSF

Preservative: PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCAMKL1

Background: CL1,CLICK1,DCAMKL1,DCDC3A,DCLK,DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

Molecular Weight:

Calculated MW: 106 kDa

Observed MW: 105 kDa

Gene ID: 9201

UniProt: O15075