Dual Specificity Phosphatase 3 (DUSP3) (Active) Protein

Catalog No. ABIN7318027

This Recombinant protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Dual Specificity Phosphatase 3 (DUSP3) (Active) Protein (ABIN7318027)

Target: Dual Specificity Phosphatase 3 (DUSP3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human DUSP3/VHR Protein (Active)

Sequence: Met 1-Pro 185

Characteristics: A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by hydrolysis of 250 μM 3-O-methyl fluorescein phosphate (OMFP).The specific activity is >1.0 μmol/min/m

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Dual Specificity Phosphatase 3 (DUSP3)

Alternative Name: DUSP3/VHR

Target Type: Viral Protein

Background:

Background: Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases.

Synonym: Dual specificity protein phosphatase 3,DUSP3,Dual specificity protein phosphatase VHR,Vaccinia H1-related phosphatase,VHR

Molecular Weight: 20.6 kDa

UniProt: P51452

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades