DYRK3 Protein (His-GST)

Catalog No. ABIN7317092

Recombinant DYRK3 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for DYRK3 Protein (His-GST) (ABIN7317092)

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This DYRK3 protein is labelled with His-GST.

Purpose: Recombinant Human DYRK3/REDK Protein (His & GST Tag)

Sequence: Met 1-Ser 588

Characteristics: A DNA sequence encoding the human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 22 nmol/min/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0

Preservative: PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Alternative Name: DYRK3/REDK

Background: DYRK5,RED,REDK,hYAK3-2,Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains oneprotein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

Molecular Weight:

Calculated MW: 93.5 kDa

Observed MW: 80 kDa

Gene ID: 8444

UniProt: O43781

Pathways: Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha