ERN1 Protein (AA 465-977) (His-GST)

Catalog No. ABIN7317060

This Recombinant ERN1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for ERN1 Protein (AA 465-977) (His-GST) (ABIN7317060)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 465-977

Purification tag / Conjugate: This ERN1 protein is labelled with His-GST.

Purpose: Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)

Sequence: Pro 465-Leu 977

Characteristics: A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 5 nmol/min/mg using MBP as substrate

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1/IRE1

Background: IRE1,IRE1P,IRE1a,hIRE1p,The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Molecular Weight:

Calculated MW: 86.0 kDa

Observed MW: 90 kDa

Gene ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response