EPH Receptor A7 Protein (EPHA7) (His tag)

Catalog No. ABIN7195538

Recombinant EPH Receptor A7 protein expressed in HEK-293 Cells.

Quick Overview for EPH Receptor A7 Protein (EPHA7) (His tag) (ABIN7195538)

Target: EPH Receptor A7 (EPHA7)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This EPH Receptor A7 protein is labelled with His tag.

Purpose: Recombinant Rat EphA7/EHK3 Protein (His Tag)(Active)

Sequence: Met1-Ser539

Characteristics: A DNA sequence encoding the rat EPHA7 (P54759)(Met1-Ser539) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Immobilized rat EPHA7-His at 10 μg/ml (100 μl/well) can bind mouse EFNA4-Fc, The EC50 of mouse EFNA4-Fc is 10.0-23.4 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EPH Receptor A7 (EPHA7)

Alternative Name: EphA7/EHK3

Background:

Background: Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines, however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.

Synonym: EPHA7,Ehk-3,Ehk3

Molecular Weight: 58.9 kDa

UniProt: P54759

Pathways: RTK Signaling