EPH Receptor B3 Protein (EPHB3) (His tag)

Catalog No. ABIN7195552

This Recombinant EPH Receptor B3 protein is expressed in HEK-293 Cells.

Quick Overview for EPH Receptor B3 Protein (EPHB3) (His tag) (ABIN7195552)

Target: EPH Receptor B3 (EPHB3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 94 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This EPH Receptor B3 protein is labelled with His tag.

Purpose: Recombinant Mouse EphB3/HEK2 Protein (His Tag)(Active)

Sequence: Met 1-Thr 537

Characteristics: A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EphB3/HEK2

Background:

Background: Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Synonym: AW456895,Cek10,Etk2,HEK2,MDK5,Sek4,Tyro6

Molecular Weight: 57 kDa

NCBI Accession: NP_034273

Pathways: RTK Signaling