FCGR2B Protein (His tag)

Catalog No. ABIN7195687

This Recombinant FCGR2B protein is expressed in HEK-293 Cells.

Quick Overview for FCGR2B Protein (His tag) (ABIN7195687)

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This FCGR2B protein is labelled with His tag.

Purpose: Recombinant Mouse CD32/FCGR2B Protein (His Tag)

Sequence: Met 1-Arg 217

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FCGR2B (NP_001070657.1) precursor was expressed with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized mouse FCGR2B-His (CD32) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 0.13-0.29 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: CD32/FCGR2B

Background: AI528646;CD32;F630109E10Rik;Fc[g]RII;FcgRII;Fcgr2;Fcgr2a;Fcr-2;Fcr-3;Ly-17;Ly-m20;LyM-1;fcRII,Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

Molecular Weight:

Calculated MW: 21.7 kDa

Observed MW: 35-40 kDa

NCBI Accession: NP_001070657

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling